Influence of gel irrigant agents in oral biofilm

Admin Dental Press

Edition V07N02 | Year 2017 | Editorial Original Article | Pages 21 to 25

Bruno Cavalini Cavenago, Samuel Lucas Fernandes, Fábio Del Mônaco Avelar, Rodrigo Ricci Vivan, Marco Antonio Hungaro Duarte

Introduction: The aim of this study was to evaluate the antimicrobial activity of 1% sodium hypochlorite, 2% chlorhexidine and 24% EDTA, all in the gel form on oral biofilm. Methods: Sterile bovine dentin blocks were allocated on an intraoral device that was used by a volunteer for 3 days. After biofilm formation, the blocks were immersed in 100 μl of evaluated substances, for 5 minutes. After treatment, the samples were stained with 50 μl of a solution of SYTO 9 / propidium iodide and evaluated in a confocal microscope, yielding 50 images per group immediately after removing the antimicrobial agent. Data were analyzed by Kruskal- Wallis and Dunn’s tests (α = 0.05). Results: Statistical differences between experimental groups and the control were verified. The 1% sodium hypochlorite was more effective than other evaluated substances (p < 0.05). The 2% chlorhexidine significantly reduced the percentage of live cells, in comparison to EDTA gel (p < 0.05). Conclusions: The irrigant agents used in this study showed no ability to dissolve induced in situ biofilm. However the sodium hypochlorite gel showed better results than chlorhexidine and EDTA. Biofilms, Dentin, Microscopy, confocal,

Cavenago BC, Fernandes SL, Avelar FDM, Vivan RR, Duarte MAH. Influence of gel irrigant agents in oral biofilm. Dental Press Endod. 2017 May-Aug;7(2):21-5. DOI:

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