Antimicrobial action of photodynamic therapy, in human root canals contaminated with Enterococcus faecalis: in vitro study

V08N02 | 2018 | Original Article | Pages 53 to 61

Leticia Ferralhi, Natanael Henrique Ribeiro Mattos, Katia Zoghbi Ospedal, Roseli Melo Bergamo, Vinicio Hidemitsu Goto Hirai, Bianca Cavalcante de Leão, Camila Paiva Perin

Photochemotherapy, Methylene Blue, Enterococcus faecalis,

INTRODUCTION: Although we have promising methods for cleaning and disinfecting in Endodontics, none of them have yet been able to completely eliminate microorganisms present in the root canal system. The advent of laser therapies, associated with specific dyes, has shown promising results in reducing root canal microbiota. OBJECTIVE: The aim of this study was to evaluate the antimicrobial action of photodynamic therapy (PDT) associated with methylene blue photosensitizer, as complementary to conventional chemical-mechanical debridement in human teeth infected with Enterococcus faecalis in vitro. METHODS: A total of 60 human single-rooted permanent teeth were prepared, infected with Enterococcus faecalis and divided into three groups of 20 each. G1 and G2: negative and positive controls, using the same specimens; G3: irrigation with 1% sodium hypochlorite (NaOCl) + 17% ethylenediaminetetracetic acid (EDTA); G4: irrigation with 1% NaOCl + 17% EDTA + PDT. Absorbent paper points were used to collect the material present in root canals, transferred to blood agar plates and cultured at 37 °C for 48 hours. In the negative control group, no samples showed contamination. RESULTS: Groups 2, 3 and 4 differed statistically from each other in relation to the mean of bacterial growth, with the lowest growth occurring in G4. CONCLUSION: Photodynamic therapy, when used as a complementary procedure to endodontic therapy, was effective against Enterococcus faecalis, thus suggesting its clinical applicability.

Ferralhi L, Mattos NHR, Ospedal KZ, Bergamo RM, Hirai VHG, Leão BC, Perin CP. Antimicrobial action of photodynamic therapy, in human root canals contaminated with Enterococcus faecalis: in vitro study. Dental Press Endod. 2018 May-Aug;8(2):53-61. DOI: https://doi.org/10.14436/2358-2545.8.2.053-061.oar

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